Proteins of the murine C-type RNA tumour viruses: isolation of a group-specific antigen by isoelectric focusing.
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چکیده
منابع مشابه
A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen
Prostate-specific antigen (PSA) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a CM-Sephadex C-50 and by gel filtration on a Sephacryl S-200 column. A single 33-kDa protein band appeared in SDS-PAGE. High pressure liquid chromatography (HPLC) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...
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The major internal protein of the RD-114 virus that appeared in a human tumor cell line, RD, after passage through fetal cats was purified by isoelectric focusing and used to prepare antibody in guinea pigs. The protein has a molecular weight of 33,500 in sodium dodecyl sulfate-polyacrylamide gels, and an isoelectric point of 9.1. This contrasts with a molecular weight of 25,000 and an isoelect...
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ورودعنوان ژورنال:
- The Journal of general virology
دوره 8 1 شماره
صفحات -
تاریخ انتشار 1970